You can purchase fresh sprouts in many health food stores including Whole Foods markets.Fresh sprouts can be stored, refrigerated for at least a week and up to two if they are properly stored. .

Complementary and alternative medicinal effects of broccoli sprouts

The effectiveness of high-sulforaphane broccoli sprouts powder, as both complementary and alternative treatment, was compared to the standard triple therapy on Helicobacter pylori eradication in type 2 diabetic patients. .

The Best Simple Broccoli Substitutes

Broccoli is one of my favourite vegetables but when I run out I use these broccoli substitutes.But there are also tender stem varieties (also called baby broccoli or broccolini) which have long edible stems and smaller florettes on top.Chinese broccoli looks like the long tender stem varieties.The Best Broccoli Substitutes.While the flavour of cauliflower is milder and less green, the shape and texture is really similar.If you’re feeding someone with an aversion to green vegetables, cauliflower can be an excellent broccoli substitute.In places where you are roasting broccoli, brussels sprouts are a brilliant substitute.If you are using broccoli as a green vegetable, kale can work really well in all applications except broccoli ‘rice’.As per kale, cooked leafy greens like spinach, collards and chard (silverbeet) can sub in beautifully when using broccoli as a side.Won’t work for roast broccoli or broccoli ‘rice’ though.Green beans.Will work in raw / steamed / boiled / roasted recipes.Don’t try to make asparagus ‘rice’ – I tried to grate asparagus with my food processor once and ended up with a slimy mess.The smaller you chop the easier they are to eat.I like to toss my raw broccoli in a salad dressing and let it stand for 30 minutes or so to soften slightly before eating.Cover with foil and roast at 220C (420F) for 15 minutes.I have had organic broccoli from the farmers market last for 2 weeks but this is rare!I eat it every week.Delicious Broccoli Substitutes Flavour Pairings.And that they are big strong dinosaurs eating the tiny trees. .

Broccoli Sprouts

This Web site is not to be used as a substitute for medical advice, diagnosis or treatment of any health condition or problem.Memorial Sloan Kettering Cancer Center makes no warranties nor express or implied representations whatsoever regarding the accuracy, completeness, timeliness, comparative or controversial nature, or usefulness of any information contained or referenced on this Web site. .

Better Boost From Broccoli Sprouts?

They’re sold in the local farmers’ market, and the seller told me that they’re 10 times as nutritious as mature broccoli.A team at Johns Hopkins found that a daily serving of broccoli sprouts reduces a marker for the presence of H. pylori by 40 percent.The researchers noted that eight weeks after the participants in their study stopped eating broccoli sprouts, the marker for H.

pylori returned to pre-treatment levels.The FDA warns against eating raw sprouts because some outbreaks of E. coli or salmonella have been traced to contaminated seeds.


Aqueous Extract of Glucoraphanin-Rich Broccoli Sprouts Inhibits

We have previously shown that sulforaphane not only inhibits formation of advanced glycation end products (AGEs) but also exerts anti-inflammatory effects on AGE-exposed human umbilical vein endothelial cells (HUVECs) and AGE-injected rat aortae.Five percent broccoli sprout extract inhibited the formation of AGEs, reduced basal gene expressions of monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1,) and receptor for AGEs (RAGE), and upregulated endothelial nitric oxide synthase (eNOS) mRNA levels in HUVECs.TNF-α upregulated MCP-1, ICAM-1, and RAGE mRNA levels in HUVECs, all of which were attenuated by the treatment with 1% broccoli sprout extract.We, along with others, have previously shown that sulforaphane inhibits the formation of AGEs in vitro and attenuates inflammatory reactions in AGE-exposed endothelial cells and AGE-infused rat aortae partly by reducing the expression of RAGE [10–12].So, in this study, we examined the effects of aqueous extract of a precursor of sulforaphane, glucoraphanin-rich broccoli sprouts on AGE formation in vitro and then investigated whether the extract could attenuate the inflammatory reactions in tumor necrosis factor-α (TNF-α)- or AGE-exposed human umbilical vein endothelial cells (HUVECs).Broccoli sprout extract was diluted with phosphate-buffered saline or cell culture medium to make final concentrations of 1% and 5% broccoli sprout extract.BSA (25 mg/ml) was incubated with 1 mM glyceraldehyde in the presence or absence of the indicated concentrations of broccoli sprout extract for 1 day, and then levels of AGEs were measured with enzyme-linked immunosorbent assay as described previously [19].HUVECs obtained from Lonza Group Ltd. (Basel, Switzerland) were cultured in endothelial basal medium supplemented with 2% fetal bovine serum, 0.4% bovine brain extracts, 10 ng/ml human epidermal growth factor, and 1 μg/ml hydrocortisone according to the manufacturer’s recommendation.After washing the cells with phosphate-buffered saline, cells were incubated with 100 μg/ml AGEs or 100 μg/ml nonglycated BSA in the presence of 1 μM carboxy-H 2 DFFDA for 25 minutes, and then fluorescence intensity was measured.We first examined the effects of broccoli sprout extract on formation of AGEs.As shown in Figure 1, Incubation of BSA with 1 mM glyceraldehyde for 1 day increased the formation of AGEs, which was significantly inhibited by 5%, but not 1% broccoli sprout extract.However, 1% of broccoli sprout extract did not affect these gene expressions except for ICAM-1; basal ICAM-1 mRNA levels were decreased to 40% of control cells by the treatment with 1% broccoli sprout extract (Figure 2(b)).We next investigated the effects of 1% broccoli sprout extract on MCP-1, ICAM-1, and eNOS gene expressions in TNF-α-exposed HUVECs.We further examined the effects of broccoli sprout extract on RAGE gene expression in HUVECs.Moreover, 1% broccoli sprout extract significantly inhibited the TNF-α-induced upregulation of RAGE mRNA levels in HUVECs (Figure 4(b)).Although AGEs elicited ROS generation in vehicle-pretreated HUVECs, the ROS-inducing effect of AGEs was not observed in 1% broccoli sprout extract-pretreated HUVECs (Figure 4(c)).Based on our previous finding that 25 μM, but not 0.4 μM sulforaphane, inhibited the formation of AGEs in vitro [12], the inhibitory effects of 5% broccoli sprout extract on AGE formation could be attributed largely to its high content of sulforaphane.We found here that 5% broccoli sprout extract for 4 hours decreased basal gene expression levels of MCP-1, ICAM-1, and RAGE in HUVECs by 50-90%, while it increased eNOS mRNA levels to 1.5-fold over the control values.However, since the effects of 5% broccoli sprout extract on nontreated HUVECs were drastic, we chose the concentration of 1% broccoli sprout extract in the experiments of TNF-α - or AGE-exposed HUVECs.In the present study, we also found that TNF-α-induced upregulation of MCP-1, ICAM-1, and RAGE mRNA levels and was significantly attenuated by 1% broccoli sprout extract.Given that MCP-1, ICAM-1, and RAGE gene expressions are transcriptionally regulated by NF-κB [10, 23], 1% broccoli sprout extract could reduce basal and TNF-α-induced MCP-1, ICAM-1, and RAGE mRNA levels in HUVECs via inhibition of NF-κB activation, which may be dependent on sulforaphane-induced Nrf2 activation.Furthermore, we have previously shown that 1.6 μM sulforaphane suppresses oxidative stress generation and inflammatory reactions in AGE-exposed HUVECs as well [10].Moreover, quercetin and sulforaphane act additively to inhibit ROS generation and MCP-1 and RAGE gene expression in AGE-exposed HUVECs [12].In this study, 1% broccoli sprout extract not only reduced RAGE mRNA levels in both nontreated and TNF-α-treated HUVECs but also blocked the ROS generation elicited by AGEs.Therefore, broccoli sprout extract may attenuate the activation of AGE-RAGE pathway by at least two mechanisms: one is the inhibition of AGE formation and the other is the suppression of RAGE expression.In this study, we could not clarify active components in broccoli sprout extract that might be responsible for the observed effects.However, the aim of the present study is to clarify whether aqueous extract of sulforaphane-rich broccoli sprout could inhibit the AGE-RAGE axis and exhibit anti-inflammatory effects in HUVECs as in the case of sulforaphane [10, 12].Our present study suggests that glucoraphanin-rich broccoli sprout extract may exert beneficial actions against vascular injury via several mechanisms, such as the inhibition of AGE formation, suppression of inflammatory reactions, reduction of RAGE expression, and upregulation of eNOS mRNA levels. .

Comparative Study of Predominant Phytochemical Compounds and

Qualitative HPLC analysis of broccoli sprouts revealed chlorogenic, p-coumaric, ferulic, gentisic and sinapic acids, and also robinin, with traces of myricetin, luteolin, quercetin and apigenin.The results (Table 1) revealed vast differences in the amounts of the phenolic acids of the tested broccoli sprouts and florets extracts.The results of Pająk et al. [20] indicated similar qualitative phenolic acids profile, but with quantitative differences: ferulic (7.66 mg/100 g dw), p-coumaric (2.04 mg/100 g dw) and chlorogenic (11.49 mg/100 g dw) acids were in lower, while sinapic acid (548 mg/100 g dw) was in higher amounts, in comparison to our results.For broccoli florets, our results are similar to those by Fernández-León et al.

[21] for sinapic acid in some Monaco cultivars.Table 1 Content of phenolic acids, flavonoids, sulforaphane and fatty acids profile of dry broccoli sprouts and florets Full size table.The content of SF is given in Table 1.Broccoli sprouts were significantly richer in SF than the florets, and the results are consistent with Nakagawa et al. [23], who indicated 10 times higher amount of SF in the sprouts in comparison to mature plants.The cytotoxic effect of broccoli florets was stronger for both cancer cell lines in comparison to the sprouts.Such effects may result from the differences in phytochemical content of both products.Cytotoxic activity of broccoli sprouts was previously studied on human leukemic cell line (HL-60), with no significant effects on proliferation or viability [31] and on two murine prostate cancer cell lines, AT2 and Mat-Ly-Lu, with interesting effect, correlated with antioxidant activity [22].Broccoli florets extracts were tested on human ovary (OVCAR-5), breast (MCF-7), colon (Colo-205) and prostate (PC-3) cancer cells [23] and significant effect on human lung cancer cells A-549 was also described [32].Strong cytotoxic effect of broccoli florets on SW480 cells, described above, proved to be due to necrosis (46.6%) rather than apoptosis (32.7%), while the opposite effect was observed for HepG2 (20.9 and 51.9%, respectively) and for normal fibroblasts (2.1 and 51.9%, respectively).On the contrary, the tested broccoli sprouts, with minor cytotoxic effect, stimulated apoptosis in SW480 (77.4%) and necrosis (50%) in HepG2 cancer cells, and their slight impact on normal fibroblasts was due to proapoptotic effect (20.9%).Second principal component in HPCA was solely loaded by second principal component from X2 block predominantly influenced by NECRO-BJ and NECRO-HEPG2. .

Broccoli Sprouts Products Delivery or Pickup Near Me

Broccoli sprouts have a flimsy white stem and very small green leaves.Broccoli sprouts also have high amounts of fiber, which can help you feel full, and they are very low in calories, making a perfect addition to a healthy diet. .


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